MEDICAL LAB TECHNICIAN EXAM KIT 1Welcome to your MEDICAL LAB TECHNICIAN STUDY MODE KIT 1NameBusinessEmailPhone NumberHow does the secondary immune response differ from the primary immune response?IgG is the predominant antibody class produced in the secondary immune responseThe antibody levels produced are higher in the secondary immune responseThe lag phase (the time between exposure to antigen and production of antibody) is shorter in the secondaryThe primary immune response typically does not involve specific IgG antibodyAll of the aboveHintThe "spiked" erythrocytes included in the image to the right may be found in each of the following conditions EXCEPT:Cardiac valve prosthesisa-beta-lipoproteinemiaDisseminated intravascular coagulationExtensive burnsHintAnalytical sensitivity of a method generally refers to:Its ability to measure only what is being assayedIts ability to measure high concentrations of analytesIt not being affected by interfering substancesIts ability to measure low concentrations of analytesHintWhich one of the following statements about Coxiella burnetii is not trueIt causes Q feverIt has a worldwide distributionIt is an obligate intracellular parasiteIt is best diagnosed by culture of the organismHintWhich of the following dematiaceous fungal species produce conidia in clusters?Alternaria speciesExophiala speciesCladosporium speciesHintA urine production of less than 400 mL/day is:Consistent with normal renal function and water balanceTermed isosthenuriaDefined as oliguriaAssociated with diabetes mellituscaused by moderate dehydrationHintThe iodine prep is least helpful in the identification of which of the following parasitic stages:LarvaeEggsCystsTrophozoitesHintA peripheral blood smear with many myeloid cells was presented for morphology review (see image on the right). Toxic granulation and vacuoles in the neutrophil most likely represent which of the following conditions?SepticemiaViral infectionTuberculosisHintStandard precautions means that:Known infectious patients must be handled with extraordinary careMask, gloves and gowns should be worn in all hospital patient's roomsAll specimens must be handled as if they are hazardous and infectiousAlways wash your hands before drawing a patients blood sampleHint The image on the right represents the result of a fluorescent antinuclear antibody (ANA) test. What pattern should be reported? Note: (a) points to the nuclei of several interphase cells, the primary consideration for discerning the ANA pattern and (b) indicates a metaphase mitotic cell. Observing the chromosomal area and cytoplasm of the metaphase cell may assist in identification of the ANA patternHomogeneousSpeckledNucleolarCentromereHintThe curved, multi-celled, dark brown staining macroconidia divided by transverse septa that can be observed in the photomicrograph on the right is characteristic of which of the following species of dematiaceous fungi?Bipolaris speciesStemphylium speciesCurvularia speciesHintIndia Ink is used to :Visualize flagellaVisualize shapeVisualize capsuleVisualize cytoplasmHintHow many grams of sodium hydroxide are required to prepare a 200 ml solution of a 10% (weight per volume) solution? (Atomic weights: Na = 23; 0 = 16; H = 1)4g10g20g40 g80 gHintWhich of the following packed red blood cell ABO types would be appropriate for transfusion to an O- negative patient?O negative only.AB negative and O negative only.AB negative, A negative and B negative only.AB negative, A negative, B negative and O negative only.HintHemoglobinopathies can be caused by all of the following structural defects EXCEPT:Deletion of a globin chain.Substitution of amino acids in a globin chainDeletion of an amino acid in a globin chainAddition of an amino acid in a globin chainFusion of globin chains.HintTable VIIICreatinine levels in mg/dL for 21 healthy hospital employees.87.98.931.04.86.901.051.08.84.971.12.95.961.021.01.93.91.98.99.941.04 What is the median of these data?.95 mg/dL.96 mg/dL.97 mg/dL.98 mg/dLHintWhat type of follow-up testing is recommended for a Speckled ANA pattern?NoneTesting for antibodies to the extractable nuclear antigens (ENA)Testing for antibodies to dsDNAHintA manual white blood cell count was performed by the hematology technologist. The cell counts for both sides were 38 and 42 respectively. All nine large squares were counted on each side. The dilution for this kit was pre-measured at 1:10. What should the technologist report as the white cell count?4.8 x 10^9/L4.4 x 10^9/L.48 x 10^9/L0.44 x 10^9/LHint_______________ is predominantly associated with skin and soft tissue infections (SSTIs), such as abscesses, cellulitis, folliculitis and impetigoHospital-associated MRSACommunity-associated MRSAHintThe trophozoite state of the intestinal flagellate Giardia lamblia is characterized by:two nucleian undulating membranefour flagellapattern of the karyosomespoked-wheel nucleoliHintWhich of the following would be the most appropriate temperature for long term storage of viral cultures:4° C-20° C-70° CRoom temperatureHintYou are performing a manual antibody titer in a clinical laboratory. You have made serial dilutions of a patient’s plasma sample and you are looking for anti-streptolysin O antibodies. The first tube contains pure patient plasma. The second tube contains a 1:2 dilution of the first tube. The third tube contains a 1:2 dilution of the second tube and so on. The patient’s antibodies no longer give visible reaction in tube 5, indicating their titer is tube 4 (or the last tube where there is a positive result). What is the concentration of antibodies in tube 4 compared to the concentration in tube 1?The concentration of antibodies in tube 4 is the same as the concentration of antibodies in tube 1The concentration of antibodies in tube 4 is about 1/4 the concentration of antibodies in tube 1The concentration of antibodies in tube 4 is 1/8 the concentration of antibodies in tube 1The concentration of antibodies in tube 4 is 4 times the concentration of antibodies in tube 1HintWhich color(s) are associated with biohazardous containers?Red and orangeBlueGreenYellowHintWhich of the following is responsible for humoral response:NeutrophilsBasophilsB lymphocytesMonocytesHintMCV is calculated using which of the following parameters:RBC histogramRBC and HctRBC and MCHCHint Identify the object contained in the cell in this illustration indicated by the arrow:Auer rodsDohle bodiesHeinz bodiesCabot ringHint Jane Doe is a 39-year-old female who felt tired for several months, had pain in the joints of her fingers, and recently developed a dermatitis following exposure to the sun. The following test results were obtained on a blood sample drawn during the initial evaluation: Total Protein = 8.4 gm/dL (N = 6.0-8.0 gm/dL) ANA >1:2560; speckled pattern CRP = positive C3 = 40 mg/dL (N = 80-180 mg/dL) C4 = 5 mg/dL (N = 15-45 mg/dL) Based on the clinical and laboratory findings, what disease should be suspected?Rheumatoid arthritisSLESjogren's syndromeSclerodermaHintThe turbidity of the bacterial inoculum of a standard disc diffusion (Kirby-Bauer) susceptibility test is:0.1 McFarland Standard0.5 McFarland Standard1.0 McFarland Standard2.0 McFarland StandardHintWhat is present in the blood of an individual with the Bombay phenotype which will cause it to agglutinate with any non-Bombay individual's blood?Anti-ABAnti-OAnti-AAnti-HHintAt what phase of antibody screen testing is it most important to read the reactions in order to detect clinically significant antibodies?AHGImmediate spin37°any of the aboveHintIf a drug is given at intervals that are the same as its half-life, approximately how long will it take the drug to reach steady state?It will be at steady state from the first doseIt will take about two half-lives to reach steady state.It will take about 5 half-lives to reach steady state.It will never reach a steady state.HintSafety Data Sheets (SDS) shall be maintained and be available to employees in the:Housekeeping DepartmentNursing OfficePersonnel DepartmentWorkplace areaSafety CabinetHintHydrogen ion concentration (pH) in blood is usually determined by means of which of the following electrodes?SilverGlassPlatinumPlatinum-lactateSilver-platinumHintWhat is the first thing you would do before you handle or open a chemical:Read the labelAsk your supervisor for helpRead the SDSall the manufacturerHintA blood specimen has been collected from an inpatient. When should the specimen be labeled?Immediately after collection in the presence of the patient.Immediately upon returning to the laboratory.Specimen tubes should be prelabeled before beginning blood collection proceduresAt any time if it is the only patient specimen being collectedHintIf the antigen frequencies for K = 0.09 and Fya = 0.66, what percent of type-specific units would be compatible for a patient with anti-K and anti-Fya?10433192100HintIn the context of the planning process, the term “goal” has been defined as a:plan for reaching certain objectivesset of specific tasksset of short and long-term plansmajor purpose or final desired resultHintA WBC differential count on CSF should be performed using the following technique:Wet mount of sample from collection tubeStained smear of a cytocentrifuged specimenDirectly from hemocytometer chamber countDirectly from stained hemocytometer countHintThe 5' end of a DNA strand ends with a OH group.TrueFalseHintIn a PCR amplification method, which of the following is amplified?TargetSignalProbeHintIllustrated in the top photograph are dry, chalky, heaped colonies growing on Middlebrook agar with a distinctive yellow-orange pigmentation. They were recovered after 5 days incubation from a subcutaneous lesion of the foot from a South American field laborer. Illustrated in the Acid-fast stain in the lower photomicrograph are slender, branching acid-fast bacilli. Casein and tyrosine were hydrolyzed. With these observations and reactions, select from the multiple choices the species identification of this isolate. Nocardia asteroidesNocardia brasiliensisMycobacterium fortuitumStreptomyces speciesHintWhich of the following techniques should be used to free someone who is "frozen" to a live electrical line?Grab the victim with bare hands and immediately and pull them awayPull the electrical line away from the victimUse a non-conductive material like wood or plastic to separate the victim from the lineThrow a blanket over the victimHintThe term used to describe patients with absence of Rh antigens is:RhdRhnullRhmodRhoHintHIPAA stands for:Health Insurance Portability and Accountability ActHealth Information Protection and Access ActHomecare Information Protection and Accountability ActNone of the AboveHintPatients who develop severe sepsis or septic shock commonly have __________ plasma lactic acid values.DecreasedNormalIncreasedHintCharcot-Leyden crystals in stool may be associated with an immune response and are thought to be the breakdown products of:neutrophilseosinophilsmonocyteslymphocytesbasophilsHintClassic automated blood cell counters are based on:Laser scatterRadio frequencyLight absorptionImpedance principleHintA patient is seen at his physician's office for scaling, flat lesions on his back that are irregular with white blotches of discoloration. The image shown is of a tease mount taken from the skin, stained with Periodic Acid Schiff, which reveals narrow fungal hyphae (arrows) along with 3 – 5 um in diameter, irregular sized spherical budding yeast cells, a presentation often referred to as “spaghetti and meatballs”. From the multiple choices, select the name of the fungus causing this infection. Hansenula anomalaMalassezia furfurAureobasidium pullulansSaccharomyces cerevisiaeHintThe most common rapid slide test (MONOSPOT©) for infectious mononucleosis employs:Horse erythrocytesSheep erythrocytesIntact beef erythrocytesNone of the aboveHintA 1:100 dilution of a patient peripheral blood was made and 136 Platelets were counted in 5 small RBC counting blocks of a Neubauer-ruled hemocytometer. What is the Platelet count?27 x 103/µL68 x 103/µL136 x 103/µL680 x 103/µL13.6 x 103/µLHintWhat has happened in an antibody titer, if tubes #5-7 show a stronger reaction than tubes #1-4?poor techniquepostzone reactionprozone reactionequivalence reactiontechnical errorHint Below is a listing of two commonly used terminology systems for genes and antigens. Use this information to answer this question: If a recipient has anti-c, which donor unit should be selected? Wiener Fisher-Race (haplotype) RoDce R1DCe R2 DcE R DCE r dce r’ dCe r” dcE ry dCEr'rRoR1R2ryr'ryRoRoHintA rosette test to screen for FMH is contraindicated if the newborn is weak-D positive :TrueFalseHintThis suspicious form was recovered in blood. Please identify the species listed below that matches the parasites shown in the image with arrows. Plasmodium vivaxPlasmodium malariaePlasmodium falciparumPseudoparasiteHintWhich one of the following tests should be used to monitor a patient's response to treatment for syphilis:TPI titerRPR titerTPHA titerFTA-ABS titerHintExtrinsic cell characteristics are reflected by the forward and side scatter on a flow cytometry cytogram.TrueFalseHintWhich immune elements are involved in a POSITIVE TB skin test?IgET cells and macrophagesNkcells and IgG antibodiesB cells and IgM antibodiesEpithelial skin cellsHintA technologist performing an antibody identification in the blood bank has recorded the following results found in the worksheet below. Which antibody(ies) are MOST LIKELY the cause of the agglutination reactions in this patient's sample?Anti-JkbAnti-kAnti-LebAnti-JkaAnti-EAnti-CAnti-cHintWhich of the following kappa / lambda ratios is found in normal serum:4 : 13 : 12 : 11 : 1HintThe border to border extension of the mycelium as illustrated in the top photograph is characteristic of one of the Zygomycetes. Although a light yellow green pigmentation of the mycelium is observed, the identification of the isolate depends on the presentation of the fruiting body observed in the bottom photomicrograph. From these observations, select the name of this isolate from the list of multiple choices : Rhizopus speciesSyncephalastrum speciesMucor speciesAbsidia speciesHintWhich of the following is NOT required to be in a machine-readable format on a blood component label :ABO & Rh of the donorProduct codeCollection facilityOutdateHintAll of the following statements about fluorometry are true EXCEPT:Fluorometry is more sensitive than spectrophotometry.Both excitation and emission spectra are characteristics of the analyteFluorescence is indirectly proportional to the analyte being measuredional to the analyte being measured Fluorometers typically utilize monochromators or optical filtersHintWhen should a prewarm technique be performed?If you suspect a warm autoantibodyIf you suspect a cold antibodyIf you suspect multiple IgG antibodiesHintA zinc deficiency in the elderly is often caused by:Decreased intake and absorptionDecreased intake and excretionIncreased intake and excretionIncreased excretion and decreased absorptionHintAbnormal crystals that can be found in urine include:LeucineTyrosineCystineTriple phosphateHintA hapten can BEST be described as :A carrier molecule for an antigen that is not antigen aloneA determinant capable of stimulating an immune response only when bound to a carrier proteinAn immunoglobulin functional only in the presence of complementHalf of an immunoglobulin moleculeHintAspirin (salicylic acid) affects platelet function by:inhibiting cyclooxygenaseinhibiting lipidsinhibiting carbohydratesactivating nucleic acidactivating Factor VIIIHintGranulocyte Concentrates MUST be administered within ______ of collection.12 hours24 hours3 days30 days35 daysHintThe renal threshold is best described as:Concentration at which a substance in the blood spills into urineConcentration at which reabsorption first occursConcentration at which kidney can no longer filter the bloodConcentration at which kidney failure beginsHintAt what frequency should quality control testing be performed on each lot of anti-human globulin to be in compliance with the FDA’s current good manufacturing practices requirements?A. At the beginning of each work shiftB. Each day of useC. Only at the time of receiptD. By each new userHintDelayed hemolytic transfusion reactions (DHTR) usually occur within which time period?5 hours after transfusion24 hours after transfusion3-10 days after transfusionone year after transfusionHintThree of the fungi that are listed below have been reported to cause the majority of opportunistic infections in humans. Select the name of the one that can be excluded from this group.Aspergillus fumigatusMucor speciesScopulariopsis speciesCandida albicansHintBordet-Gengou cough plates are used to isolate:Mycobacterium tuberculosisCorynebacterium diphtheriaeBordetella pertussisHaemophilus aegyptiusHaemophilus ducreyiHintThe image on the right is a cytospin preparation of cerebrospinal fluid (CSF). What is the blood cell type that is indicated by the arrow?Segmented neutrophilMonocyteEosinophilMacrophageHintIn this photomicrograph are observed several background dematiaceous hyphae within which is seen a long, flask-shaped, tapered phialide (arrow) that has a flat saucer-like terminus. This feature is most characteristic of:Phialophora verrucosaPhialophora richardsiaeExophiala jeanselmeiScopulariopsis brumptiiHintWhat is the primary target of HIV?HeartLiverLungsT-helper cellsHintIf a patient has an antibody to a low-frequency antigen like Kpa or Jsa, how would this impact the ability to find compatible blood and to identify the antibody?Difficult to find compatible blood because everyone has the antigen and difficult to identify the antibodyDifficult to find compatible blood but easy to identify the antibodyEasy to find compatible blood but difficult to identify the antibodyEasy to identify the antibody and easy to find compatible bloodHintSpherocytes are associated with which two of the following conditions:Hereditary spherocytosisAutoimmune hemolytic anemiaThalassemiaIron deficiencyHintWhich of the following immunoglobulin classes is chiefly responsible for the degranulation of mast cells and basophils:IgGIgAIgMIgEHintThe cytospin technique perfectly preserves the morphology of blood cells in a fluid sample.TrueFalseHintWhich of the following can lower the amount of current needed to cause electricity-induced injury?Ground wiresWet skinMore carbon dioxide in the airAll the aboveHintWhich one of the following blood groups usually reacts LEAST strongly with anti-H?BA2A1HintAn urticarial transfusion reaction is characterized by:Rapid rise in temperatureDifficulty breathingRash and hivesBlood in the urineHintWhen given during pregnancy, RhIg may cross the placenta and cause a positive DAT in the newborn.TrueFalseHintWhen administering Fresh Frozen Plasma, which one of the following is considered standard blood bank practice?Should be ABO compatible with the recipient's red blood cellsMust be the same Rh type as the recipientIs appropriate as a volume expanderComponent should remain frozen when it is issuedHintWhich of the following serological test results may occur in a patient experiencing a hemolytic transfusion reaction due to antibodies such as anti-c and anti-K? (Select all that apply.)Patient's antibody strength may initially decrease.Patient may develop a positive DAT with mixed-field agglutination.Patient's antibody becomes undetectable.Patient's antibody increases in titer weeks later.HintWhat is the most prudent step to follow to select units for cross-match after recipient antibodies have been IDENTIFIED?perform DAT and IAT on patient cells and donor unitsantigen type patient cells and any donor cells to be cross-matchedadsorb any antibodies from the patient serumobtain a different enzymes for repeat antibody panel testingcross match several additional units just in caseHintWhich is in the correct order from the lowest concentration of H antigen to the highest concentration of H antigen?Bombay, A1B, A1, A2B, B, A2, OA1, O, B, A2, Bombay, A1B, A2BBombay, O, A1B, A2, A1, B, A2BA1B, A2B, A2, O, B, A1, BombayHintWhich listed transfusion reaction is MOST OFTEN associated with transfused patient's lacking IgA?HemolyticAnaphylaxisFebrileTransfusion-associated circulatory overload (TACO)Hint Red blood cells with a positive DAT cannot be tested accurately with blood typing reagents that require an indirect antiglobulin technique unless they have been treated with ___________________ to dissociate IgG from the RBC membrane.Chloroquine diphosphateFicinZZAPAny of the aboveHintWhich of the following conditions is most frequently associated with anti-I:Cold agglutinin diseaseHemolytic transfusion reactionDelayed transfusion reactionInfectious mononucleosisHintWhich one of the following statements about directed donations is true:They are truly safer than random donor unitsThey will never cause graft-versus-host diseaseThey do not cause logistic problems for the blood bank and transfusion serviceThey are often perceived by the recipient or his family as safer than random donor unitsHintUnits of A-, B-, and O-negative red blood cells are shipped to your transfusion service. What testing MUST be performed by your facility prior to placing these units into your inventory?ABOABO and RhABO, Rh and antibody screenABO, Rh and Weak D to confirm negative statusHBVHintFor transfusion services in the United States, which of the following incidents must be reported to the Food and Drug Administration (FDA) because of a biological product deviation?Incident A: A unit was issued with an incorrect expiration date. The expiration date was incorrectly marked as one day earlier than the actual expiration date.Incident B: A nurse in ICU misidentified the patient and initiated the transfusion of Rh positive blood to an Rh negative patient.Incident C: The wrong specimen was used to crossmatch a unit and the unit was issued.All of the aboveHintFor which of these reasons would a molecular method be used to determine a pregnant woman's Rh type?To determine if she is homozygous or heterozygous for DTo determine if she is Rh negativeTo differentiate between weak D or partial DHintA former patient had an anti-E four years ago, but her antibody panel is now negative. Since she now needs blood for surgery, what should the blood bank do?get autologous blood from relativescrossmatch E-negative unitsgive group O negative whole bloodgive random compatible unitsHintWhich of the following statements best describes Rh antibodies:Naturally occurring, IgMImmune, IgGNaturally occurring, IgGImmune, IgMHintA group A Rh positive infant born to a group O Rh negative woman has a 1+ positive DAT. The mother had a negative antibody screen at delivery and received antenatal RhIg. Which of the following are possible causes of the newborn's positive DAT?Anti-APassive anti-DAntibody to a low frequency antigenAll of the above are possible causesNone of the above are possible causesHintWhich is the best component to treat a patient with fibrinogen deficiency?Red Blood CellsPlatelet ConcentrateFresh Whole BloodCryoprecipitated AHFHintA patient experiences a mild allergic reaction to a transfusion, including urticaria, erythema (skin redness), and itching. What is the most likely source of the allergen?Plasma proteinsLeukocyte antibodiesPlatelet antibodiesDonor Red Blood CellsHint A mother's serologic results are shown above. Her newborn types as group A Rh positive with a (1+) positive direct antiglobulin test (DAT). Which of the following investigative tests would be most useful to resolve the cause of the positive DAT and should be done FIRST?Test an eluate prepared from newborn's red cells against an antibody identification panel by IAT.Test newborn's plasma against group A1 red cells and group O antibody screen cells by IAT.Test newborn's plasma against mother's red cells by IAT.Test newborn's plasma against father's red cells by IAT.HintWhen AHG or Coombs serum is used to demonstrate that red cells are antibody coated in vivo, the procedure is termed:Indirect techniqueDirect techniqueHemagglutination techniqueHemolysis techniqueHintWhich of the following blood groups reacts least strongly with Anti-H:OA2A1A1BHintWhich of the following antigens is classified as a Major Histocompatibility Complex Class II antigen (MHCII)?HLA-AHLA-BHLA-CHLA-DRHintAn acute hemolytic reaction may be caused by which of the following? (Choose all that apply)Transfusion of ABO incompatible blood.Failure to irradiate cellular products.Exposure to a red cell antigen when the corresponding antibody is present.Leukocyte antibodiesHintWhich of the following red blood cells contain the most H antigen:Group A cellsGroup B cellsGroup O cellsBombay phenotypeHintWhich unit contains the proper number of platelets from a random donor?3.8 x 10104.0 x 10104.5 x 10105.8 x 1010HintWhich of the procedures listed below will increase the platelet concentration in the preparation of platelets?Centrifuge the blood at a low speed, remove the plasma and spin the plasma again at a low speed.Centrifuge the blood at a low speed, remove the plasma and spin the plasma again at a high speed.Centrifuge the blood at a high speed, remove the plasma and spin the plasma again at a high speed.Increase the duration of rotationHintAt 28 weeks gestation, a group A Rh-negative female had a negative antibody screen and was injected with Rh immune globulin (RhIg). Anti-D is detected in the mother's serum at delivery. A panel is performed that excludes the presence of other antibodies. The most likely conclusion would be passive anti-D due to RhIg administration.TrueFalseHintThe ability to produce anti-D varies significantly among Rh negative individuals.TrueFalseHintWhich of the following blood group antigen-antibody reactions is enhanced by using enzymes:FyaFybMJkaHintWhich Rh antibody might be produced if a unit of blood with Rh genotype DCe/dce is given to a patient with Rh genotype DCe/DCe?Anti-CAnti-cAnti-dAnti-ceAnti-fHintIf an Rh negative patient is administered a unit of R1R1 packed red cells, which one of the following antibodies would be most likely to develop:Anti-cAnti-EAnti-eAnti-DHintWhich of the following tests are suitable for quantifying the size of fetomaternal hemorrhage (FMH)? Select all that apply.Flow cytometryKleihauer-Betke testRosette testWeak D (microscopic Du)testHint Time's upSubmit a Comment Cancel replyYour email address will not be published. Required fields are marked *Comment *Name * Email * Website Save my name, email, and website in this browser for the next time I comment.